The two teams sit level on seven points at the top of Group H after three games ahead of Los Blancos' first ever visit to Wembley.
Here, Sportsmail tells you all you need to know about the European showdown.
Tottenham players make their way out for training ahead of their clash with Real Madrid
Cristiano Ronaldo (centre) and Co in action as Madrid trained at Wembley on Tuesday
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Tottenham vs Real Madrid team news
In-form striker Harry Kane is expected to return to Spurs' starting XI having missed their last two matches with a hamstring injury.
The Englishman took full part in the team's open training session on Tuesday with Pochettino going to to say he was 'very confident' his star man would be passed fit for the European showdown.
'He's on the list,' The Tottenham boss said when asked about Kane's availability in his pre-match press conference. 'Maybe it's possible for him to play but I don't want to say 100 per cent'.
'He trained but it is important we make the right decision,' Pochettino added. 'With the doctors and the player, we will take the best decision for everyone. We must analyse and take the best decision for everyone.'
In-form striker Harry Kane is expected to return to Spurs' starting XI after a hamstring injury
Elsewhere, Kane's compatriot Dele Alli will make his first Champions League appearance of the season after serving a three-game suspension.
Kenyan midfielder Victor Wanyama is the only first-team regular ruled out as he continues his rehabilitation from a knee injury.
As for the visitors, Zinedine Zidane opted to play his strongest team in Sunday's trip to Girona but that didn't prevent a shocking 2-1 defeat.
The defeat wasn't the only blow as Raphael Varane now misses Wednesday's clash after being forced off at half-time with a muscular issue.
Former Spurs hero Gareth Bale also misses out despite returning to training from a calf problem whilst Keylor Navas and Dani Carvajal remain on the sidelines.
Isco has been named in the travelling squad despite missing training on Monday.
Former Spurs hero Gareth Bale also misses out despite returning to training from a calf
Spurs vs Real possible line-ups
Tottenham: Lloris; Alderweireld, Sánchez; Vertonghen; Aurier, Dier, Dembélé, Davies; Eriksen, Alli; Kane
Real Madrid: Casilla; Achraf, Ramos, Nacho, Marcelo; Casemiro, Kroos, Modrić, Asensio; Ronaldo, Benzema
Champions League standings in Group H
As it stands: Real Madrid (7), Tottenham (7), Borussia Dortmund (1), APOEL Nicosia (1)
Matches: Tottenham vs Real Madrid, Borussia Dortmund vs APOEL Nicosia
It could be an excellent night to be a Spurs fans, and not so great if you're of a Dortmund persuasion.
Tottenham and Real know that victory for either club in their crunch Wembley showdown will secure their place in the last 16.
Should the two teams draw on Wednesday night, either APOEL or Dortmund could be knocked out with a defeat in the group's other game.
Mauricio Pochettino is looking to secure qualification for the last-16 with two games to spare
Wembley TV coverage and time
The match will be televised live on BT Sport 2, with coverage starting from 7pm. BT Sport subscribers will be able to stream the match online via the video player and the BT Sport app.
Alternatively, you can follow all the build-up and action from 7pm with Sportsmail's live blog.
Tottenham vs Real Madrid Odds
Real Madrid 6/5
Ronaldo and Co have been made favourites for the Champions League clash at Wembley
Source : http://www.dailymail.co.uk/sport/football/article-5038857/Spurs-vs-Real-Madrid-Team-news-line-odds.html